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abckit by vectastain  (Vector Laboratories)


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    Vector Laboratories abckit by vectastain
    Abckit By Vectastain, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abckit+by+vectastain/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Goat+IgG)/pmc07008286-111-16-19
    Average 94 stars, based on 426 article reviews
    abckit by vectastain - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice
    Article Snippet: .. Step 3: incubation with secondary antibody and peroxidase complex : the slides were washed in PBS and incubated with the secondary antibody using an ABCKit by Vectastain (Vector Elite-PK-6105 (anti-goat)/PK-6101 (anti-rabbit)). .. Step 4: visualization : the slides were washed in PBS and proteins were visualized using 3,3′-diaminobenzidine chromogen (DAB) (Sigma Chemical Co., St. Louis, Missouri, USA).

    Article Title: Effects of Anti-IL-17 on Inflammation, Remodeling, and Oxidative Stress in an Experimental Model of Asthma Exacerbated by LPS.
    Article Snippet: .. The following day, the slides were washed in PBS and incubated with a secondary antibody using ABCKit by Vectastain (Vector Elite-PK-6105 anti-goat), PK-6101 (anti-rabbit), and PK-6102 (anti-mouse). .. For visualization of positive cells, the slides were washed in PBS and proteins were visualized using 3,3’-diaminobenzidine chromosome (DAB) (Sigma Chemical Co., St. Louis, MO, USA).

    Article Title: A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice
    Article Snippet: Immunohistochemistry was performed with the following anti-bodies:: anti-mouse macrophage Mac2 (Cedarlane Lab, Burlington, ON, Canada; 1:60,000), anti-mouse neutrophils (AbD Serotec, Kidlington, UK; 1:400), anti-mouse MMP-9 (Santa Cruz Biotechnology, Dallas, TX, USA; 1:500), antibody anti-mouse MMP-12 (Santa Cruz Biotechnology, 1:100), anti-iNOS (LabVision, NeoMarkers, Fremont, CA, USA; 1:500), anti-eNOS (LabVision, NeoMarkers; 1:100), anti-TIMP-1 (LabVision, NeoMarkers; 1:400), anti-TNF (Santa Cruz Biotechnology; 1:300,), anti-isoprostane-8 (Oxford Biomedical Research, Oxford, UK; 1:10,000), and anti-MUC5AC (LabVision, NeoMarkers; 1:400). .. Next, the slides were washed in PBS and incubated with secondary antibody using an ABCKit by Vectastain (Vector Elite-PK-6105 (anti-goat) or PK-6101 (anti-rabbit) (Vector Laboratories, Burlingame, CA, USA). .. For visualization of the positive cells the slides were washed in PBS and proteins were visualized using 3,3′-diaminobenzidine chromogen (DAB) (Sigma Chemical Co., St. Louis, MO, USA).

    Article Title: Virgin Coconut Oil Supplementation Prevents Airway Hyperreactivity of Guinea Pigs with Chronic Allergic Lung Inflammation by Antioxidant Mechanism
    Article Snippet: Blocking of endogenous peroxidases was performed by incubation with 3% hydrogen peroxide (H 2 O 2 ) 10 V (3 × 10 min), and sections of experimental and control (positive and negative) tissue slides were incubated overnight with the anti-iNOS, dilution 1 : 1600 (IS-20 Goat Polyclonal; Oxford Biomed, Resear, MI, USA) or anti-8-iso-PGF2 α , dilution 1 : 1500 (N-32020 Mouse Monoclonal; BD transduction Lab., CA, USA). .. The following day, the slides were washed in PBS and incubated with a secondary antibody using ABCKit by Vectastain (Vector Elite-PK-6105 anti-goat) or PK-6102 (anti-mouse). .. For visualization of positive cells, the slides were washed in PBS, and proteins were visualized using 3,3′-diaminobenzidine chromosome (DAB) (Sigma Chemical Co., St. Louis, MO, USA).

    Article Title: Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice
    Article Snippet: The sections were incubated overnight at 4°C with anti-TNF-α (1:400; R&D Systems, Minneapolis, MN, USA), anti-IL1β (1:400), anti-IL6 (1:200), anti-IL8 (1:200), anti-IL10 (1:300), anti-IL17 (1:250), anti-CD4+ (1:600), anti-iNOS (1:400), anti-forkhead box P3 (FOXP3) (1:1200), anti-p65-NF-κB (1:250), anti-8-iso-PGF2α (1:1500), anti-TGF-β (1:600), anti-MMP-9 (1:500), anti-MMP-12 (1:450), anti-TIMP-1 (1:100), anti-actin (1:1600), anti- ROCK 1 (1:50), and anti-ROCK 2 (1:100; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA). .. The following day, the slides were washed in PBS and incubated with a secondary antibody using the ABCKit by Vectastain (Vector Elite-PK-6105 anti-goat), PK-6101 (anti-rabbit), and PK-6102 (anti-mouse). .. For visualization of positive cells, the slides were washed in PBS and proteins were visualized using 3,3'-diaminobenzidine chromosome (DAB; Sigma Chemical Co., St. Louis, MO, USA).

    Article Title: Effects of Anti-IL-17 on Inflammation, Remodeling, and Oxidative Stress in an Experimental Model of Asthma Exacerbated by LPS
    Article Snippet: .. The following day, the slides were washed in PBS and incubated with a secondary antibody using ABCKit by Vectastain (Vector Elite-PK-6105 anti-goat), PK-6101 (anti-rabbit), and PK-6102 (anti-mouse). .. For visualization of positive cells, the slides were washed in PBS and proteins were visualized using 3,3’-diaminobenzidine chromosome (DAB) (Sigma Chemical Co., St. Louis, MO, USA).



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